Figure 7

The effect of Notch3 inhibiting EMT can be counteracted by knocking down Kibra with shRNA. (a–d) After MDA-MB-231 cells were first transfected with pCLE/N3 for 48 h, Kibra expression was knocked down through a second transfection with shKibra #5–#8. RT-PCR results reveal that the effect of Notch3 inhibiting EMT was significantly attenuated at the transcriptional level as demonstrated by the expression of Notch3, Kibra, E-cadherin and slug mRNA. (e) MDA-MB-231 cells were subjected to the above-mentioned co-transfection. Kibra expression was knocked down through transfection with shKibra #5–#8. The effect of Notch3 inhibiting EMT was significantly attenuated at the protein level as demonstrated via western blotting to test the expression of Notch3, Kibra, E-cadherin and vimentin. *P<0.05, **P<0.01 and ***P<0.001 (Student’s t-test) as compared with control cells. Data are presented as mean±s.e.m. (n=3)