Figure 2
From: FOXK1 interaction with FHL2 promotes proliferation, invasion and metastasis in colorectal cancer
Interaction between FHL2 and FOXK1 proteins in CRC cells. (a) Double staining of FHL2 and FOXK1 in SW480 cells with Hoechst by confocal microscopy. (b) Western blotting analysis of FOXK1 and FHL2 expression in the indicated CRC cells. (c) PCI-flag-FHL2 plasmid was transfected into SW480 and SW620 cells. Immunoprecipitation was performed with anti-flag antibody, and pre-immune normal mouse immunoglobulin G (nm IgG) was used as control. Western blotting was performed with anti-FOXK1 antibody. The IP blot was probed with indicated antibodies to show the input of whole-cell lysates. IP, immunoprecipitation; Wb, western blot. (d) Cell lysates of SW480 and SW620 cells were immmunoprecipitated by anti-FOXK1 antibody or the control antibody, normal rabbit immunoglobulin G (nr IgG). Western blotting was carried out with anti-FHL2 antibody. The IP blot was probed with indicated antibodies to show the input. All these experiments were repeated two to three times with similar findings. Scale bars represent 20 μm in a.
