Figure 1
From: In vivo functional dissection of a context-dependent role for Hif1α in pancreatic tumorigenesis

Hif1α is downregulated in murine PDAC cells. (a) Western blot analyses show the downregulation of Hif1α in shHif1α cells but not in shControl cells: (left) western blot result; and (right) quantitative measurement; shHif1α: transfection with Hif1α-specific shRNA-expressing vector; shControl: transfection with control vectors. (b–d) The metabolic state of shControl cells or shHif1α cells is determined by glucose uptake assay (b), glutamate assay (c) and lactate secretion assay (d). (e) Vegfa secretion between shControl and shHif1α cells is measured by Vegfa ELISA assay. (f) The proliferation capacity of shControl or shHif1α cells is determined by colony formation assay. All data are presented as mean±s.e.m. (n=3), unpaired t-test is used to examine statistical significance, *P<0.05. See Supplementary Materials and Methods.