Figure 2
From: In vivo functional dissection of a context-dependent role for Hif1α in pancreatic tumorigenesis

Hif1α depletion promotes primary tumour growth with tissue necrosis in vivo. (a) The orthotopic transplantation experiment shows the volume of tumours derived from shControl and shHif1α cells-injected animals: (left) gross pathology; and (right) quantitative measurement. (b) Representative H&E staining pictures show larger necrotic regions in shHif1α tumours compared with shControl tumours. (c) Immunohistochemistry (IHC) stainings of cleaved-caspase 3 (left) demonstrate more apoptotic cells (middle) and larger non-necrosis area (right) in shHif1α tumours in comparison with shControl tumours. (d) Western blot (left) and quantification results (right) show increased expression of cleaved-caspase 3 in shHif1α cells under hypoxic conditions. (e) IHC staining of phosph-histone H3 (pH-H3) and quantitative analysis demonstrates increased proliferation in shHif1α tumours; scale bar, 100 μm. See Supplementary Materials and Methods.