Figure 2 | Oncogenesis

Figure 2

From: In vivo functional dissection of a context-dependent role for Hif1α in pancreatic tumorigenesis

Figure 2

Hif1α depletion promotes primary tumour growth with tissue necrosis in vivo. (a) The orthotopic transplantation experiment shows the volume of tumours derived from shControl and shHif1α cells-injected animals: (left) gross pathology; and (right) quantitative measurement. (b) Representative H&E staining pictures show larger necrotic regions in shHif1α tumours compared with shControl tumours. (c) Immunohistochemistry (IHC) stainings of cleaved-caspase 3 (left) demonstrate more apoptotic cells (middle) and larger non-necrosis area (right) in shHif1α tumours in comparison with shControl tumours. (d) Western blot (left) and quantification results (right) show increased expression of cleaved-caspase 3 in shHif1α cells under hypoxic conditions. (e) IHC staining of phosph-histone H3 (pH-H3) and quantitative analysis demonstrates increased proliferation in shHif1α tumours; scale bar, 100 μm. See Supplementary Materials and Methods.

Back to article page