Figure 1
AURKA expression is inversely correlated with gastric cancer prognosis and overrides DNA damage-induced cell death. (a) Western blot analysis of AURKA expression in gastric cancer (T) and normal adjacent tissues (N). Whole-tissue extracts were prepared and western blotting analysis performed with the indicated antibodies. GAPDH was used as internal loading control. (b) Kaplan–Meier survival analysis of 240 gastric cancer patients. Patients’ primary tissues were grouped by high and low AURKA staining values. High AURKA expression was significantly associated with the overall survival (OS) rate. (c) Multivariate cox regression analysis of disease-free survivals (DFS) and OS. 95% CI, 95% confidence interval; HR, hazard rate. (d) BGC823 cells transfected with cDNA encoding wild-type AURKA were treated with or without doxorubicin (1 μg/ml) for 48 h and subjected to western blot and TUNEL assay. Data represent means±s.e.m. of three independent experiments with significance determined by one-way ANOVA followed by Tukey’s multiple comparison test. (e) BGC823 cells transfected with AURKA siRNA sequences were treated with or without doxorubicin (1 μg/ml) for 48 h and subjected to western blot and TUNEL assay. Data represent means±s.e.m. of three independent experiments with significance determined by one-way ANOVA followed by Tukey’s multiple comparison test.
