Figure 2

PRH and pPRH are present in normal prostate, BPH and prostate adenocarcinoma. Representative immunohistochemistry from normal prostate (a, b), BPH (c and d), and prostate adenocarcinoma (e, f) stained for total PRH using the M6 monoclonal antibody at 1:200 dilution (a, c, e) and pPRH using the YKN5 antibody at 1:750 dilution (b, d, f) as described in the text, and counterstained using Mayers haematoxylin. (g, h) Normal tissue stained using mouse and rabbit IgG, respectively (× 40 magnification). (i) A summary of the immunohistochemistry data for pPRH (YKN5) and PRH (M6) staining presented as the percentage of cells/nuclei that stain and the intensity of staining. AC, adenocarcinoma (with Gleason score); NA, not available.