Figure 5
Effect of VS-6063 and JQ1 on ovarian tumor cell survival. (a, b) Flow cytometry-based analyses of Annexin V and PI-positive cells in response to treatment of VS-6063 and JQ1. OVC-8 or OVC-433 cells were treated with inhibitors over 48 h, detached and stained with propidium iodide and APC-conjugated Annexin V, and quantified by flow cytometry (left panels). Values (right panels): Mean±s.e., n=3. (c) Analyses of PARP1 cleavages in tumor cells treated with varying doses of VS-6063 and JQ1. Tumor cells were lysed in RIPA buffer, and immunoblotted with antibody against PARP1 or β-actin (as a control).
