Figure 2
LZAP depletion downregulates exogenously expressed p53 and attenuates p53 induction and transactivation in response to DNA damage. (a) LZAP and ectopically expressed wild-type or mutant p53 R175H protein levels (left) or relative to GPDH mRNA levels (right) in p53 null Saos-2 cells expressing control or LZAP siRNA. mRNA levels were determined using qRT-PCR; mean from two experiments is shown, error bar represents s.d.; P-values were calculated using unpaired t-test. (b) Immunoblot detecting LZAP and p53 in HCT116 cells infected with retrovirus containing control or shRNA specific to LZAP prior to treatment with zeocin (200 μg/ml) for the indicated times (left); right: fold change in the expression of p53 pro-apoptotic transcriptional targets in HCT116 cells stably control or LZAP shRNA after zeocin treatment (200 μg/ml) for 24 h, as measured by qRT-PCR. Mean from three experiments is shown, error bar represents s.d.
