Figure 3 | Oncogenesis

Figure 3

From: Loss of LZAP inactivates p53 and regulates sensitivity of cells to DNA damage in a p53-dependent manner

Figure 3

LZAP depletion protects wild-type p53-expressing cells from DNA damage, while sensitizes mutant p53 cells to the treatment. (a) U2OS parental and LZAP CRISPR cells were plated (1000 cells per well of 96 well plates) prior to treatment with the indicated DNA-damaging agents. Six days later, viability was measured using Cell Titer Glo (Promega). (b) HCT116 stable LZAP knockdown cells (or control) or UNC10 parental, or LZAP CRISPR cells (c) were treated with zeocin for 6 days prior to viability analysis. Mean is shown, error bars represent s.d., N=3; P-values were calculated using paired t-test.

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