Figure 5 | Oncogenesis

Figure 5

From: Loss of LZAP inactivates p53 and regulates sensitivity of cells to DNA damage in a p53-dependent manner

Figure 5

LZAP depletion affects cells survival after DNA damage in a p53-dependent manner. (a) Relative to GPDH mRNA levels of LZAP or TP53 in U2OS or U2OS LZAP CRISPR cells transiently transfected with psuper vectors expressing control or p53 shRNAs as determined on qRT-PCR. (b) Survival after increasing concentrations of zeocin of cells from a as determined by methylene blue staining, extraction and absorbance measurement, 7 days after the treatment. (c) Relative to GPDH CDKN1A expression in p53 null cells Saos-2 transfected with control or LZAP siRNAs and wild-type or mutant p53, as determined by qRT-PCR; mean from two experiments is shown, error bar represents s.d.; P-values were calculated using unpaired t-test. Survival after increasing doses of zeocin of Saos-2 cells-co-transfected control or LZAP siRNA and wild-type or mutant p53 (d), or Saos-2 cells transfected or not with control or LZAP siRNAs (e), P-values were calculated using paired t-test.

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