Table 2 Oligonucleotide sequences: oligonucleotides for confirming LZAP mice genotype (5′-TGTGCCACCACGCAACTTTT-3′; 5′-CATGAAGACAGAACCAAAC-3′)

From: Loss of LZAP inactivates p53 and regulates sensitivity of cells to DNA damage in a p53-dependent manner

qRT-PCR oligonucleotides

Gene

Sequence (5′-3′)

H. sapiens CDK5RAP3 F

CAATGCTGCCATCCAGGACATG

H. sapiens CDK5RAP3 R

ATCCGCTGTGAAGAGTATCGGC

H. sapiens TP53 F

CCTCAGCATCTTATCCGAGTGG

H. sapiens TP53 R

TGGATGGTGGTACAGTCAGAGC

H. sapiens CDKN1A F

AAGACCATGTGGACCTGT

H. sapiens CDKN1A R

GGTAGAAATCTGTCATGCTG

H. sapiens PPM1D F

GAAGAAACTGGCGGAATGG

H. sapiens PPM1D R

TTGTGAGTGAGTCGAGGTCGT

H. sapiens BAX F

TGGAGCTGCAGAGGATGATTG

H. sapiens BAX R

GAAGTTGCCGTCAGAAAACATG

H. sapiens APAF1 F

GCTCTCCAAATTGAAAGGTGAAC

H. sapiens APAF1 R

ACTGAAACCCAATGCACTCC

H. sapiens BBC3 F

GCAGGCACCTAATTGGGCT

H. sapiens BBC3 R

ATCATGGGACTCCTGCCCTTA

H. sapiens PIDD F

TCTGACACGGTGGAGATGTTCG

H. sapiens PIDD R

AGGTGCGAGTAGAAGACAAAGCAG

  1. Abbreviations: H. sapiens, homo sapiens; LZAP, leucine zipper-containing ARF-binding protein; qRT-PCR, quantitative reverse transcriptase real-time PCR.
  2. Oligonucleotide sequences used for confirming mouse genotype and for qRT-PCR.
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