Figure 2
From: Oncogenic MCT-1 activation promotes YY1-EGFR-MnSOD signaling and tumor progression
MCT-1 overexpression protects cells against oxidative stress via YY1. MCF-10A cells (control and MCT-1) with YY1 knockdown (shYY1) or scrambled knockdown were studied. (a) EGFR and p53 expression were analyzed in cells with different MCT-1 and YY1 levels. The protein amounts were normalized to GAPDH and compared with scrambled control (lane 1). (b) Relative EGFR promoter activity is indicated. (c) Relative EGFR mRNA level is shown. (d) Relative p53 promoter activity is shown. (e) After the cells were exposed to 100 μm H2O2 for 24 h, apoptotic events were evaluated using FITC-Annexin V staining and propidium iodide (PI) counter staining, followed by flow cytometry analysis. The data represent the mean±s.d. (n=3). ***P<0.001.
