Figure 1 | Oncogenesis

Figure 1

From: MAGE-TRIM28 complex promotes the Warburg effect and hepatocellular carcinoma progression by targeting FBP1 for degradation

Figure 1

TRIM28 interacts with FBP1 in HCC cells. (a) Sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS–PAGE) and silver staining of proteins purified by tandem affinity purification from 293T cells transfected with control vector or SFB-tagged FBP1. (b) Western blot analysis of reciprocal co-immunoprecipitation of ectopically expressed Flag-FBP1 and HA-TRIM28 in 293T cells. (c) Western blot analysis of reciprocal co-immunoprecipitation of endogenous FBP1 and TRIM28 proteins in HepG2 cells. (d) Schematic diagram depicting a set of GST-TRIM28 recombinant protein constructs. (e) Western blot analysis of FBP1 proteins in HepG2 whole-cell lysate pulled down by GST or GST-TRIM28 recombinant proteins. (f) Schematic diagram depicting a set of GST-FBP1 recombinant protein constructs. (g) Western blot analysis of TRIM28 proteins in HepG2 whole-cell lysate pulled down by GST or GST-FBP1 recombinant proteins.

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