Figure 2
Mint3 in MEFs promotes cancer cell proliferation in a HIF-1-dependent manner. (a) Illustration of co-culture experiments. (b, c) Secreted luciferase activity from GLuc-expressing MDA-MB-231 (b) and A431 cells (c) co-cultured with MEFs. (d) Western blot analysis of mock, FLAG-tagged Mint3 and FLAG-tagged MT1-MMP expression in Mint3 KO MEFs. (e, f) Secreted luciferase activity from GLuc-expressing MDA-MB-231 (e) and A431 cells (f) co-cultured with mock, Mint3 or MT1-MMP expressing Mint3 KO MEFs. (d) HIF-1α knockdown in MEFs was confirmed by qRT–PCR. (g, h) Secreted luciferase activity from GLuc-expressing MDA-MB-231 (e) and A431 cells (f) co-cultured with control or HIF-1α siRNA-treated MEFs. (i) FIH-1 knockdown in MEFs was confirmed by western blotting. (j, k) Secreted luciferase activity from GLuc-expressing MDA-MB-231 (h) and A431 cells (i) co-cultured with control or FIH-1 siRNA-treated MEFs. In b,c,e–i,k and l, error bars indicate the s.d. (n=3). The data were analysed using a t-test. *P<0.05, **P<0.01. The data shown are representative of three independent experiments with similar results. NS, not significant; qRT–PCR, quantitative RT–PCR.
