Figure 6
Mint3 in CAFs promotes cancer cell proliferation and tumour growth. (a) Western blot analysis of Mint3, L1CAM, MT1-MMP and FIH-1 expression in NFs and CAFs. (b) Western blot analysis of L1CAM and MT1-MMP expression in CAFs transfected with control (CTR) or MT1-MMP siRNA. (c) Western blot analysis of L1CAM and Mint3 expression in control (shLacZ) or Mint3 knockdown (shMint3) CAFs. (d, e) Secreted luciferase activity from GLuc-expressing MDA-MB-231 (d) and A431 cells (e) co-cultured with control or Mint3 knockdown CAFs. (f, g) Representative photographs (left panel; day 25) and growth rate (right panel) following subcutaneous injection of MDA-MB-231 (f) and A431 cells (g) with or without indicated CAFs in immunodeficient mice. In d and e, error bars indicate the s.d. (n=3). The data were analysed using a t-test. **P<0.01. The data shown in a–e are representative of three independent experiments with similar results. In f and g, error bars indicate the s.e.m.; n=12 from two independent experiments (n=6 and n=6, respectively); the data shown were analysed using the Mann–Whitney U-test. *P<0.05, **P<0.01.
