Figure 7
PHB expression levels are reduced and PHB protein is further dephosphorylated in bicalutamide-resistant LNCaP cells. (a) MTT assays on LNCaP (wt) and LNCaP cells with acquired bicalutamide resistance (Bic R) treated with increased doses of bicalutamide (μM) over 96 h. Data represent the average of three independent experiments. (b) Q-PCR analysis of PHB transcript levels in LNCaP Bic R cells. Levels are normalised to housekeeping genes (RPL19, GAPDH and β-actin). (c) 2D Western blot for PHB from LNCaP (wt) and LNCaP (Bic R) cells grown in media with charcoal-stripped serum for 72 h. (d) MTT assays of LNCaP (wt) cells with increasing doses of bicalutamide with or without Src inhibitor over 96 h. (e) MTT assays of LNCaP (Bic R) cells with increasing doses of bicalutamide with or without Src inhibitor over 96 h. Data represent the average of three independent experiments.
