Figure 2 | Oncogenesis

Figure 2

From: Regulation of miR-483-3p by the O-linked N-acetylglucosamine transferase links chemosensitivity to glucose metabolism in liver cancer cells

Figure 2

2-DG treatment affects miR-483-3p expression at transcriptional level. (a) miR-483-3p relative expression by RT–qPCR (left panel), and CTNNB1 (wild-type and mutated) protein relative expression by western blot (right panel), in HepG2 cells treated with 2-DG 20 mM for 2 weeks in high glucose. The graphs represent the means of technical triplicates with the respective s.d. For statistical analysis, Student’s t-test was applied (**P<0.01). The right panel shows the percentage variation with respect to the relative control by densitometry analysis. (b) Electrophoretic mobility shift assay (EMSA) of nuclear extract (NE) from HepG2 cells treated with 2-DG 5 mM using the miR-483 E-box probe. The specific complexes are indicated with black arrows (b, c). Arrow a shows the supershift generated by USF1 complex. Densitometry analysis is shown. EMSA was performed once. (c) Protein relative expression of cytoplasmic (CE) and nuclear (NE) CTNNB1 and USF1 by western blot. Densitometry analysis is shown.

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