Figure 4 | Oncogenesis

Figure 4

From: Regulation of miR-483-3p by the O-linked N-acetylglucosamine transferase links chemosensitivity to glucose metabolism in liver cancer cells

Figure 4

Effect of 2-DG and 5-FU combination in vitro. (a) Caspase 3/7 activation, normalized on ACTB protein expression measured by western blot (data not shown), and (b) Annexin V analysis of HepG2 cells treated with 2-DG (10 and 20 mM), 5-FU (2.5 μM) or in combination in Dulbecco’s modified Eagle’s medium (DMEM) low glucose for 24 h. Only bright annexin was measured as high apoptosis rate was detectable in treated cells.

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