Figure 5

Effect of 2-DG and 5-FU combination in vivo. (a) Time table of the in vivo experiment. Sixteen NSG mice were randomly divided in four groups of treatment: vehicle (PBS), 2-DG, 5-FU and 2-DG+5-FU. Weeks and days of the different treatments are reported. At day 1, the mice were injected with 10 × 10⁶ HepG2 cells. At the second, fourth and sixth week, the mice were treated with 2-DG, 5-FU, both the drugs or the vehicle. Every day of treatment, the mice received a single intraperitoneal injection of 200 μl. Every drug or combination was dissolved in PBS. In (b), the total weight of xenografts obtained by NOD scid gamma (NSG) mice is reported. (c) miR-483-3p relative expression, normalized on RNU44, by RT–qPCR from the same samples (white bars) and from HepG2 cells before inoculation in mice (black bar). The graphs represents the means of technical triplicates with the respective s.d. For statistical analysis, Student’s t-test was applied (*P<0.05; **P<0.01). (d) Protein relative expression by western blot of CTNNB1 and PUMA in tumors induced in xenografts model. Densitometry analysis is shown. Every value is normalized on relative ACTB, and it reports the percentage of difference for every group of treatment vs the control group.