Figure 2
The impact of Rb depletion on lipid class and acyl chain composition. (a) Heat map of the mean fold change in each lipid class between control MEFs and Rb-depleted MEFs. Red, increase; white, average; blue, decrease. Names of the lipid class shown in red are significantly increased, and blue are significantly decreased in Rb-depleted MEFs. Four batches of MEFs derived from one embryo were used per one shRNA. P<0.05 by Student’s t-test. (b) Levels of the indicated lipid class in control MEFs (solid) and Rb-depleted MEFs (blank). Data represent mean+s.d. (n=4) *P<0.05, **P<0.01 by Student’s t-test. (c) The total lipid content in control MEFs (solid) and Rb-depleted MEFs (blank). Data represent mean+s.d. (n=4). (d, e) Heat map represents log 2 fold changes in the indicated two different lipid species in Rb-depleted MEFs as compared to control MEFs. Red, increase; white, average; blue, decrease. (f) Heat map of the mean fold change in DAG species in Rb-depleted MEFs as compared to control MEFs (lower). Red, increase; white, average; blue, decrease. Names of the lipid class shown in red are significantly increased, and blue are significantly decreased in Rb-depleted MEFs (P<0.05 by Student’s t-test). The number of carbon–carbon double bonds (C=C) for each lipids is shown in the bar graph (upper). (g) Levels of the indicated DAG species in control MEFs (solid) and Rb-depleted MEFs (blank). Data represent mean+s.d. (n=4) *P<0.05, **P<0.01 by Student’s t-test. (h, i) Heat map represents log 2 fold changes in the different lipid species in Rb-depleted MEFs as compared to control MEFs. Red, increase; white, average; blue, decrease.
