Figure 6
Effects of inhibition of de novo MUFAs synthesis on sphere formation induced by Rb inactivation. (a) Sphere assay of RN6 cells infected with pLXSB vectors expressing the indicated proteins and selected. 2 × 103 cells were seeded and sphere images were acquired after 7 days. Scale bars, 1,500 μm (top) and 300 μm (bottom). Data represent mean+s.d. (n=4). *P<0.05, **P<0.01 by Student’s t-test. (b) Tumors developed in KSN/Slc mice transplanted with RN6 cells transduced with the indicated expression vectors and selected. 1 × 104 cells were injected subcutaneously into male KSN/Slc mice. Tumors were weighed 46 days after injection. Data represent mean+s.d. (n=4) *P<0.05, **P<0.01 by Student’s t-test. (c, d) RT–qPCR of the indicated genes in RN6 cells transduced with the indicated proteins and cultured under the indicated conditions. Data represent mean+s.d. (n=3–5). *P<0.05, **P<0.01 by Student’s t-test. (e) Sphere assay of RN6 cells infected with lentiviruses expressing the indicated shRNAs and selected. 2 × 103 cells were seeded and sphere images were acquired after 7 days. Scale bars, 300 μm. Data represent mean+s.d. (n=4). *P<0.05, **P<0.01 by Student’s t-test. (f) Tumors developed in KSN/Slc mice transplanted with RN6 cells infected with lentiviruses expressing the indicated shRNAs and selected. 1 × 104 cells were injected subcutaneously into male KSN/Slc mice. Tumors were weighed 46 days after injection. Data represent mean+s.d. (n=4) *P<0.05, **P<0.01 by Student’s t-test. MUFA, mono-unsaturated fatty acid.
