Figure 4
From: Spontaneous loss of B lineage transcription factors leads to pre-B leukemia in Ebf1+/–Bcl-xLTg mice
Pre-B lymphoma/leukemia is characterized by loss of lineage-commitment transcription factors. (a) A population of B220loCD244+ cells is present in healthy EB mouse bone marrow but not in peripheral tissues or in Bcl-xLTg or wild-type mice. Additional staining (not shown) defined this population as late pre-B: B220loCD19+BP-1−CD25+IgM− (same phenotype as EB tumor cells, far right panel). Flow cytometric analyses of total bone marrow, splenocytes, peripheral blood leukocytes (PBL) and subiliac lymph nodes are shown. Fluorochromes used for B220 were PE-Cy7 and eFluor450. Contour plots in this figure are representative of eight affected EB, four unaffected EB, five Bcl-xLTg and five Ebf1+/– control mice analyzed. (b) RT–qPCR analysis of relative transcript levels from selected genes in sorted late pre-B cells (B220+IgM–BP-1–CD25+) from the bone marrow of five congenic wild-type mice, three Bcl-xLTg mice, B220loCD244+ (presumed tumor progenitor) cells from the bone marrow of three healthy EB bone marrow transfer recipients (Unaffected EB), and leukemic cells from four different EB mice (EB tumors). *P<0.05; **P<0.01; ***P<0.001; ****P<0.0001. Primers sequences are listed in Supplementary Table S1.
