Figure 1

Expression and regulation of β-hCG by BRCA1 in breast cancer. All the experiments were done in triplicates. All error bars in the graphs represent s.d. (a) qRT-PCR of β-hCG expression in breast/ovary/prostate cancer cell lines. (b) β-hCG expression in cancer cell lines using ELISA. (c) qRT-PCR analysis of CGB5 and CGB7 expression in HCC1937 and HCC1937/wt BRCA1. Expression was normalized to HCC1937 and HCC1937/wt BRCA1, respectively. (d) β-hCG protein expression at different time points in HCC1937 and HCC1937/wt BRCA1 using ELISA. Expression of CGB5 and CGB7 in BRCA1 silenced (e) HCC1937 and (f) HCC1937/wt BRCA1 by qRT-PCR. Expression was normalized to scrambled siRNA transfected HCC1937 and HCC1937/wt BRCA1 (g) Semi-quantitative RT-PCR amplification of CGB5 promoter immunoprecipitated with anti-BRCA1 antibody using fragmented chromatins from HCC1937, SUM149 and HCC1937/wt BRCA1. Normal chromatin fragments were used as input. Chromatin samples immunoprecipitated with IgG was used as negative control. (h) qRT-PCR amplification of CGB5 promoter immunoprecipitated with anti-BRCA1 antibody and IgG in HCC1937 and HCC1937/wt BRCA1. ***P<0.001; **P<0.01; *P<0.05.