Figure 2
From: PRAS40 promotes NF-κB transcriptional activity through association with p65
PRAS40 associates with P65. (a) Plasmids expressing GST-PRAS40, GST-only, Venus-p65 or Venus only were transfected as indicated. Glutathione beads were used to precipitate GST-tagged proteins. Eluates and input lysates were analyzed by western blotting. (b) Endogenous PRAS40 was co-immunoprecipitated with p65 in melanoma A375 and lung cancer A549 cells.
