Figure 5
From: PRAS40 promotes NF-κB transcriptional activity through association with p65
Residues 180-200 of p65 are sufficient for PRAS40 association. (a) Truncations of P65 were made based on its known structure and function. RHD represents Rel Homology Domain for the function of dimerization and TAD means transcription activation domain. ✓ means could interact with PRAS40; × means could not interact with PRAS40 according to the results of a GST co-precipitation assay (b, c). (b) Left blot: Full-length and truncations of P65 (1-313, 1-521, 1-534) could be co-precipitated by PRAS40. Middle blot: p65 truncations 190-290 and 1-190 of P65 could not be co-precipitated by PRAS40. Right blot: p65 truncations 1-196 and 126-313 could be co-precipitated by PRAS40. The remaining lanes were the negative controls. (c) A Venus-tagged p65 180–200 peptide could be co-precipitated by PRAS40.
