Figure 2 | Oncogenesis

Figure 2

From: Impaired p65 degradation by decreased chaperone-mediated autophagy activity facilitates epithelial-to-mesenchymal transition

Figure 2

Regulation of p65 by chaperone-mediated autophagy. (a, b) MCF-10A and HeLa cells were stably infected with LAMP2A overexpression vector (a) or shRNA against LAMP2A (b) followed by immunoblots for indicated proteins. Left: representative images. Right: quantification analysis of the blot density of p65. *P<0.05, **P<0.01 and ***P<0.001 as compared with con. (c, d) Cell lysates from HEK293T cells were immunoprecipitated with anti-p65 (c) or anti-LAMP2 (d) antibodies. Precipitates were analyzed by western blots. (e) MCF-10A and HeLa cells were stably infected with two shRNAs against HSC70 followed by analysis of indicated proteins by immunoblots. Left: representative images. Right: quantification analysis of the blot density of p65. *P<0.05 and **P<0.01 as compared with the siren group. (f, g) Cell lysates from HEK293T cells were immunoprecipitated with anti-p65 (f) or anti-HSC70 (g) antibodies and precipitates were analyzed by western blots. (h, i) HEK293T cells were transfected with Flag-tagged p65 (h) or Flag-tagged HSC70 (i) and co-IP assay was performed with Flag M2 beads 48 h post transfection and followed by immunoblots for the indicated proteins.

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