Abstract
The number-average and weight-average molecular weights of seven samples of poly(S-[(3-hydroxypropyl)-carbamoylmethyl]-L-cysteine) (poly(Cys[CamPrOH])) have been determined by measurements of osmotic pressure of 0.1% CH3COOH solutions and light scattering of hexafluoro-2-propanol solutions, respectively. A strong tendency of poly(Cys[CamPrOH]) to form the β-conformation is observed in various solvents, including CH3COOH–H2O, DCA, H2O, and D2O. When dissolved in H2O at 70°C, poly(Cys[CamPrOH]) having molecular weight lower than 18,000 is randomly coiled and that having molecular weight higher than 20,000 is subject to the thermally-induced β-coil transition. When dissolved in D2O at 70°C, poly(Cys[CamPrOH]) undergoes the thermally-induced β-coil transition, irrespective of the molecular weight. D2O stabilizes the β-conformation more effectively than does H2O, by the change in the structure of water rather than by the substitution of D for H on the polypeptide.
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Ikeda, S., Tomiyama, T. Molecular Weight and Conformation of Poly(S-[(3-hydroxypropyl)-carbamoylmethyl]-L-cysteine) in Various Solvents. Polym J 14, 33–42 (1982). https://doi.org/10.1295/polymj.14.33
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DOI: https://doi.org/10.1295/polymj.14.33
