Abstract
The conformation and properties of porcine brain calmodulin (CaM) in egg lecithin liposome were investigated by measuring fluorescence and circular dichrorism (CD). Two to five percent CaM could be incorporated into the unilamellar vecicles and the fluorescence emmision maximum of dancylated CaM was found to shift toward shorter wavelength by 20 nm. In contrast, a capture efficiency of CaM in multilamellar vesicles was 10 to 30% and the α-helical content of CaM increased with increasing lecithin concentration. In the absence of Ca2+ at the lecithin concentration of 5–10 mg ml−1, the CD spectrum suggested the same conformation for CaM which bound Ca2+. In the presence of Ca2+ at 1–2 mg ml−1 lecithin concentration, the CD spectrum indicated the destruction of α-helical conformation, whereas, at 5–10 mg ml−1 lecithin concentration, the CD spectrum was the same with that of CaM upon calcium binding. The results of CD measurements were supported by fluorescence spectra.
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Kondo, Y., Naoi, M., Fujii, T. et al. Conformation and the Properties of Porcine Brain Calmodulin Incorporated in Liposomes. Polym J 19, 1359–1364 (1987). https://doi.org/10.1295/polymj.19.1359
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DOI: https://doi.org/10.1295/polymj.19.1359
