Abstract
The interactions of poly(γ-glutamic acid) with Cu(II) and Mn(II) ions in aqueous solution were studied by 1H and 13C nuclear magnetic resonances. The addition of Cu(II) ions to poly(γ-glutamic acid) solution selectively broadens the α-proton signal, the side-chain carboxyl carbon signal, and the α-carbon signal. The addition of Mn(II) selectively broadens the α-proton signal, the side-chain carboxyl carbon signal, and the main chain carbonyl carbon signal. It is concluded that the oxygen atom of the side-chain carboxyl group of poly(γ-glutamic acid) binds to Cu(II) and Mn(II) ions in aqueous solution at neutral pH and at room temperature. It is also probable that the nitrogen atom of the amide group binds to Cu(II) and that the oxygen atom of the carbonyl group binds to Mn(II). These groups bound to Cu(II) and Mn(II) ions undergo rapid exchange between the bound and free states, and the life time of the bound state is short, less than 10−4 s for Mn(II) complex.
Similar content being viewed by others
Log in or create a free account to read this content
Gain free access to this article, as well as selected content from this journal and more on nature.com
or
References
T. Mineshita, S. Matsumura, H. Ono, T. Watanabe, and S. Ono, Bull. Chem. Soc. Jpn., 42, 594 (1969).
D. Balasubramanian, C. C. Kalita, and J. Kovacs, Biopolymers, 12, 1089 (1973).
S. Sawa, T. Murakawa, T. Watanabe, S. Murao, and S. Omata, J. Agri. Chem. Soc. Jpn., 47, 167 (1973) (in Japanese).
D. I. Marlborough, Biopolymers, 12, 1083 (1973).
T. Hara, Y. Fujino, and S. Ueda, J. Appl. Biochem., 4, 112 (1982).
O. Iwaki, K. Hikichi, M. Kaneko, S. Shimizu, and T. Maruyama, Polym. J., 4, 623 (1973).
T. Hiraoki, M. Kaneko, and K. Hikichi, Polym. J., 11, 397 (1979).
T. Hiraoki, M. Kaneko, and K. Hikichi, Polym. J., 11, 591 (1979).
N. Higuchi, K. Kakiuchi, Y. Kyogoku, and K. Hikichi, Macromolecules, 13, 79 (1980).
N. Higuchi, T. Hiraoki, and K. Hikichi, Macromolecules, 13, 81 (1980).
T. J. Swift and R. E. Connick, J. Chem. Phys., 37, 307 (1962).
Z. Luz and S. Meiboom, J. Chem. Phys., 40, 2686 (1964).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Hikichi, K., Tanaka, H. & Konno, A. Nuclear Magnetic Resonance Study of Poly(γ-glutamic acid)—Cu(II) and Mn(II) Complexes. Polym J 22, 103–109 (1990). https://doi.org/10.1295/polymj.22.103
Issue date:
DOI: https://doi.org/10.1295/polymj.22.103
Keywords
This article is cited by
-
Poly-γ-glutamic acid, a bio-chelator, alleviates the toxicity of Cd and Pb in the soil and promotes the establishment of healthy Cucumis sativus L. seedling
Environmental Science and Pollution Research (2018)
-
Biosynthesis and chemical modification of poly(?-glutamic acid)
Polymer Bulletin (1994)
