Abstract
A series of amphiphilic block copolymers (1) containing poly[(N-acetylimino)-ethylene] as a hydrophilic block was prepared and solution properties were studied by gel-filtration method, dynamic light scattering, 1H NMR, and viscosity measurement. Except for polymers containing poly[(N-propionylimino)ethylene] as a hydrophobic segment, all polymers formed micelle-like polymer aggregates. The proportion of the aggregate fraction increased with hydophobicity of the polymer. Regardless of the structures of the hydrophobic segments, the particle sizes of the polymer aggregates fractionated by gel filtration chromatography were ca. 150 nm by dynamic light scattering. Interactions of Lipase P with the resulting polymer aggregates in water were studied by the gel-filtration method. We found all aggregates to strongly incorporate Lipase P, and enhance the hydrolysis of Lipase P. The maximum amount of Lipase P incorporated to the polymer aggregate was 4000.
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Naka, K., Nakamura, T., Ohki, A. et al. Aggregates of Amphiphilic Block Copolymers Derived from Poly[(N-acylimino)ethylene]s and Their Complexes with Lipase in Water. Polym J 27, 1071–1078 (1995). https://doi.org/10.1295/polymj.27.1071
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DOI: https://doi.org/10.1295/polymj.27.1071
