Abstract
Peptides were genetically produced that were composed of two or three repeats of the self-assembling peptide RADA16 (RADARADARADARADA), and referred to as RADA16 × 2 and RADA16 × 3, respectively. These peptides were expressed as fusion proteins that retained the activity of the fusion partner protein. The expressed peptides exhibited both fibril formation and the ability to support cell adhesive activity. Moreover, hydrogels formed by the peptides via the addition of a medium provided a three-dimensional environment for cell proliferation.
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We are grateful to Ms Keiko Yamamichi from the Technical Department of the Tokyo Institute of Technology for technical support with the transmission electron microscope imaging.
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Mie, M., Oomuro, M. & Kobatake, E. Hydrogel scaffolds composed of genetically synthesized self-assembling peptides for three-dimensional cell culture. Polym J 45, 504–508 (2013). https://doi.org/10.1038/pj.2012.216
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DOI: https://doi.org/10.1038/pj.2012.216
