Abstract
A thermo-responsive elastin, elastin-like peptide (ELP)–gold nanoparticle (AuNP) hybrid (ELP–AuNP), was successfully prepared via the in situ reduction of HAuCl4 in the presence of lipoic acid-terminated ELP: (VPGVG)4 (V:valine, P:proline, G:glycine) synthesized using a solid-phase peptide synthesis method. The conformation and aggregation properties of the ELP–AuNP in an aqueous buffer solution were examined using circular dichroism (CD) and ultraviolet-visible (UV-Vis) spectroscopies and transmission electron microscopy (TEM) observation. The phase transition of the ELP layer at the periphery of the AuNP because of the conformational change from random coil to β-turn structures was found to proceed gradually with increasing temperature and to simultaneously cause an aggregation of the ELP–AuNP because of the component’s hydrophobic interactions and a color change of the solutions from red to violet. The ELP–AuNPs were aligned selectively onto patterned, hydrophobic polystyrene stripes on a glass plate when the plate was immersed and incubated in the solution containing the ELP–AuNP above its phase transition temperature, as verified using atomic force microscopy (AFM) and scanning electron microscopy (SEM).
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Acknowledgements
This work was supported in part by a grant-in-aid for scientific research (no. 21750130), and by the Strategic Development of Research Infrastructure for Private Universities on ‘Creating a Research Center for Advanced Molecular Biochemistry’ from the Ministry of Education, Culture, Sports, Science and Technology (MEXT), Japan.
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Higashi, N., Ochiai, T., Kanazawa, C. et al. Site-specific adsorption of gold nanoparticles coated with thermo-responsive peptides. Polym J 45, 523–528 (2013). https://doi.org/10.1038/pj.2012.220
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DOI: https://doi.org/10.1038/pj.2012.220
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