Abstract
A polyethylene terephthalate polymer surface was modified by dip coating in a polymer mixture composed of methacrylate-based terpolymers containing both perfluoroalkyl (Rf) groups and poly(ethylene oxide) (PEO) side chains, vinylidenefluoride-tetrafluoroethylene copolymers ((CH2CF2)80(CF2CF2)20) and poly(methyl methacrylate). The terpolymers contained Rf groups with various numbers of carbons. After the dip coating, both the Rf groups and the PEO chains predominantly segregated on the top surface. The modified surfaces were characterized with X-ray photoelectron spectroscopy, protein adsorption, thrombogenesis and cell adhesion. The results indicate that because of the surface-segregated PEO chains, the surfaces exhibited resistance to nonspecific protein adsorption, antithrombogenicity and resistance to cell adhesion. In addition, covalent conjugation of biotin with the PEO termini enabled selective immobilization of streptavidin from a mixed protein solution on the dip-coated surface.
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References
Bernard, A., Delamarche, E., Schmid, H., Michel, D., Bosshard, H. R. & Biebuyck, H. Printing patterns of proteins. Langmuir 14, 2225–2229 (1998).
Peterbauer, T., Heiz, J., Olblich, M. & Hering, S. Simple and versatile methods for the fabrication of arrays of live mammalian cells. Lab. Chip. 6, 857–863 (2006).
Yates, J. R., Speicher, S., Griffin, P. R. & Hunkapiller, T. Peptide mass maps: a highly informative approach to protein identification. Anal. Biochem. 214, 397–408 (1993).
Schweitzer, B., Roberts, S., Grimwade, B., Shao, W., Velleca, M. & Kingsmore, S. F. Multiplexed protein profiling on microarrays by rolling-circle amplification. Nat. Biotechnol. 20, 359–365 (2002).
Yuan, X., Yoshimoto, K. & Nagasaki, Y. High-performance immunolatex possessing a mixed-PEG/antibody coimmobilized surface: highly sensitive ferritin immunodiagnostics. Anal. Chem. 81, 1549–1556 (2009).
Holmberg, K., Bergstorm, K., Osterberg, E., Tiberg, F. & Harris, J. M. Effects on protein adsorption, bacterial adhesion and contact angle of grafting PEG chains to polystyrene. J. Adhes. Sci. Technol. 7, 503–517 (1993).
Ista, L. K., Fan, H., Baca, O. & Lopez, G. P. Attachment of bacteria to model solid surfaces: oligo(ethylene glycol) surfaces inhibit bacterial attachment. FEMS Microbiol. Lett. 142, 59–63 (1996).
Lee, J. H. & Andrade, J. D. Blood compatibility of polyethylene oxide surfaces. Prog. Polym. Sci. 20, 1043–1079 (1995).
Jo, S. & Park, K. Surface modification using silanated poly(ethylene glycol)s. Biomaterials 21, 605–616 (2000).
Lee, J. H., Kopecek, J. & Andrade, J. D. Protein-resistant surfaces prepared by PEO-containing block copolymer surfactants. J. Biomed. Mater. Res. 23, 351–368 (1989).
Lens, J. P., Harmsen, P. F. H., Ter Schegget, E. M., Terlingen, J. G. A., Engbers, G. H. M. & Feijen, J. Immobilization of functionalized alkyl-poly(ethylene oxide) surfactants on poly(ethylene) surfaces by means of an argon plasma treatment. J. Biomater. Sci. Polym. 8, 963–982 (1997).
Qie, Y. X., Klee, D., Pluster, W., Severich, B. & Hocker, H. Surface modification of polyurethane by plasma-induced graft polymerization of poly(ethylene glycol) methacrylate. J. Appl. Polym. Sci. 61, 2373–2382 (1996).
Tokuda, K., Noda, M., Kotera, M. & Nishino, T. “Multifunctional Polymer Surface with Segregation of Poly(ethylene oxide) Side Chains.” Proceedings of ADHESION’13 61–65 (Society of Adhesion and Adhesives (SAA), York, UK, 2013).
Smith, P. K., Krohn, R. I., Hermanson, G. T., Mallia, A. K., Gartner, F. H., Provenzano, M. D., Fujimoto, E. K., Geoke, N. M., Olson, B. J. & Klenk, D. C. Measurement of protein using bicinchoninic acid. Anal. Biochem. 150, 76–85 (1985).
Sung, D., Park, S. & Jon, S. Facile method for selective immobilization of biomolecules on plastic surfaces. Langmuir 25, 11289–11294 (2004).
Park, I. J., Lee, S. B. & Choi, C. K. Surface properties for poly(perfluoroalkylethyl methacrylate)/poly(n-alkyl methacrylate)s mixtures. J. Appl. Polym. Sci. 54, 1449–1445 (1994).
Kassis, C. M., Steehler, J. K., Betts, D. E., Guan, Z., Romack, T. J., DeSimone, J. M. & Linton, R. W. XPS studies of fluorinated acrylate polymers and block copolymers with polystyrene. Macromolecules 29, 3247–3254 (1994).
Pillai, S., Arpanaei, A., Meyer, R. L., Birkedal, V., Gram, L., Besenbacher, F. & Kingshott, P. Preventing protein adsorption from a range of surfaces using an aqueous fish protein extract. Biomacromolecules 10, 2759–2776 (2009).
Czuha, M. Jr . & Riggs, W. X-ray photoelectron spectroscopy for trace metals determination by ion-exchange absorption from solution. Anal. Chem. 47, 1836–1838 (1975).
Tanaka, M., Motomura, T., Kawada, M., Anzai, T., Kasori, Y., Shiroya, T., Shimura, K., Onishi, M. & Mochizuku, A. Blood compatible aspects of poly(2-methoxyethyl acrylate) (PMEA)-relationship between protein adsorption and platelet adhesion on PMEA surface. Biomaterials 21, 1471–1481 (2000).
Vroman, L. & Adams, A. L. Identification of rapid changes at plasma-solid interfaces. J. Biomed. Mater. Res. 3, 43–67 (1969).
George, P. A., Donose, B. C. & Cooper-White, J. J. Self-assembling polystyrene-block-poly(ethylene oxide) copolymer surface coatings: resistance to protein and cell adhesion. Biomaterials 30, 2449–2456 (2009).
Yu, Q., Zhang, Y., Chen, H., Zhou, F., Wu, Z., Huang, H. & Brash, J. L. Protein adsorption and cell adhesion/detachment behavior on dual-responsive silicon surfaces modified with poly(N-isopropylacrylamide)-block-polystyrene copolymer. Langmuir 26, 8582–8588 (2010).
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Tokuda, K., Noda, M., Maruyama, T. et al. A low-fouling polymer surface prepared by controlled segregation of poly(ethylene oxide) and its functionalization with biomolecules. Polym J 47, 328–333 (2015). https://doi.org/10.1038/pj.2014.131
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DOI: https://doi.org/10.1038/pj.2014.131
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