Abstract
Insulin stimulates the rate of protein synthesis and has a marked effect on RNA metabolism. Previous work on diabetic insulins has shown that serum derived juvenile diabetic insulin is abnormally resistant to degradation by insulinase and that pancreatic diabetic insulin has a decreased capacity to stimulate the incorporation of glycogen into rat diaphragms. The purpose of this study was to examine the possibility that diabetic insulin is abnormal in its action on RNA synthesis. A series of cultures are inoculated simultaneously with 5 × 105 log phase diploid mouse fibroblasts of 3T6 strain. Growth is allowed until day four at which time the medium is replaced with fresh medium containing 0.5 mc of uracil-2-C14 (S.A. 20 mc/millimole). Pancreatic insulins from normal and adult diabetics were prepared by acid-ethanol extraction and added to the tissue culture in a concentration of 1000 μ units/per ml of nutrient medium. After a 48 hr. incubation period, the cellular monolayer is subdivided by conventional chemical methods and the C14 activity of the RNA fraction is estimated.
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Roy, C., Gotlin, R., Shapcott, D. et al. 15 Decreased Incorporation of Uracil-2-C14 in the RNA Fraction of Mouse Fibroblast Cultures Grown with Pancreatic Diabetic Insulins. Pediatr Res 1, 203–204 (1967). https://doi.org/10.1203/00006450-196705000-00022
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DOI: https://doi.org/10.1203/00006450-196705000-00022
