Abstract
Extract: Human metaphase cells were treated sequentially with trypsin. Photographs of the cells stained before and during this treatment showed first Giemsa (G) banding then a gradual decrease in prominence of G bands and the appearance of C bands. These results were confirmed using computer-generated density curves.
Speculation: These results are more consistent with loss or change in configuration of chromosomal protein being the primary mechanism by which G and C bands are produced than denaturation-renaturation. Unless trypsin banding is carefully controlled a combination of G and C banding will be produced.
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Merrick, S., Ledley, R. & Lubs, H. Production of G and C Banding with Progressive Trypsin Treatment. Pediatr Res 7, 39–44 (1973). https://doi.org/10.1203/00006450-197301000-00006
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DOI: https://doi.org/10.1203/00006450-197301000-00006
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