Abstract
The detailed study of the kinetics of the anticonvulsant, diphenylhydantoin (DPH), in small children has been difficult with conventional gas chromatographic (GLC) methods. A new radioimmuno-assay (RIA) developed by Cook and Christensen (Res. Comm. Chem. Path. Pharmacol. 5: 767, 1973) was duplicated in our laboratory and some of the characteristics of the assay defined. The sensitivity of this assay allowed DPH levels to be determined accurately in 20 μl of plasma. The antibody is highly specific for DPH and the assay correlates well with GLC. In several infants being treated for seizures with a commonly used dose, plasma DPH levels were found to be low. The RIA method facilitated monitoring their plasma levels. When plasma levels were measured following an IV dose of DPH, the infants were found to metabolize the drug more rapidly than did adolescents and adults.
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Johnston, M., Haslam, R. & Lietman, P. USE OF RADIOIMMUNOASSAY TO STUDY DIPHENYLHYDANTOIN PHARMACOKINETICS IN CHILDREN. Pediatr Res 8, 363 (1974). https://doi.org/10.1203/00006450-197404000-00141
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DOI: https://doi.org/10.1203/00006450-197404000-00141
