Abstract
In this communication, data are presented on the morphological changes which occurred when human skin fibroblasts were grown in medium containing serum from hypopituitary patients prior to and during treatment with human GH. Skin fibroblasts obtained from normal and GH deficient children were established in monolayer culture using F10 with 10% fetal calf serum (v/v). When these cells were subcultured for 2 passages in F10 with 10% untreated hypopituitary serum, the fibroblasts, during the second passage, exhibited strikingly abnormal cellular morphologies and disruption of monolayer growth. The cells became rounded, detached from the growing surface and floated free in the medium. The floating fibroblasts, whether derived from the control subject or GH deficient patients, re-established normal monolayer confluency when replated into medium containing normal serum or serum from hypopituitary patients during GH treatment. Morphological changes were recorded using a Unitron Inverted Photo-microscope.
This human fibroblast system provides an additional in vitro method for studying the growth promoting properties of sera from children with various forms of growth failure.
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MacGillivray, M., Hastings, C., Brown, J. et al. EFFECTS OF SERUM FROM GROWTH HORMONE DEFICIENT PATIENTS ON THE IN VITRO GROWTH OF HUMAN SKIN FIBROBLASTS. Pediatr Res 8, 371 (1974). https://doi.org/10.1203/00006450-197404000-00187
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DOI: https://doi.org/10.1203/00006450-197404000-00187
