Abstract
Lumen membranes isolated from renal cortex of fetal, 3-5 day, 3-4 week and adult rabbits were compared for trehalase specific activity(SA), D-glucose uptake and by electron microscooy(EM). Brush border membranes were immature in fetal and 3-5 day rabbits but mature in 3-4 week ones by EM. Trehalase, a disaccharidase, is a marker enzyme for tubule brush borders. Its SA expressed as μmoles/min/mg protein X 10−2, and nembrane D-glucose uptake at 5 mM expressed as nmoles/min/mg protein, were :
D-glucose uptake by membranes was examined over the range 0.5 mM-50 mM. Although the Km of glucose uptake by membranes from 3-5 day and adults was similar (24 mM & 21 mM),the Vmax was lower, 8 nmoles vs. 21 nmoles. Membrane glucose uptake was inhibited by phloridzin and N-ethyl malemide. Urine glucose fell from .67 mM in 3 day to .09 mM in 4 week rabbits. These studies indicate that: 1)trehalase SA increases 50X as brush borders develop with age, 2)glucose transport increases as brush borders mature, 3)Vmax increases as urine glucose decreases, 4)trehalase is a marker of these changes in the development of cortex luminal membranes.
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Chesney, R., Sacktor, B. & Drummond, K. DEVELOPMENTAL CHARACTERISTICS OF ISOLATED RABBIT RENAL CORTEX MEMBRANES. Pediatr Res 8, 454 (1974). https://doi.org/10.1203/00006450-197404000-00685
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DOI: https://doi.org/10.1203/00006450-197404000-00685
