Abstract
The binding capacity of TeBG was determined by polyacrylamid gel electrophoresis after preincubation of serum with 3H-dihydrotestosterone (3H-DHT). Steady state binding conditions were obtained by initial disolving of 3H-DHT in the gel. In these conditions, the binding of 3H-DHT to TeBG was displaced by addition of unlabel-led DHT. The saturation curve was determined by addition of increasing concentrations of 3H-DHT in serum incubates. At saturation, the TeBG binding capacity of serum (BC) expressed as μg p. cent ml was calculated from the peak of radioactivity. Mean ± SD BC value was 3.85 ± 0.37 in prepubertal boys and significantly lower (p<0.01) in adult men : 1.47 ± 0.6. In boys from birth to 6 months mean BC value was 3.5 ± 1.6, while it was only 1.37 ± 0.3 in cord blood. In 2 boys with precocious puberty BC values were 0.34 and 1.6, and in 3 patients with complete testicular feminisation (TF) 4.41, 4.83 and 5.90. The association of a low BC and a high plasma testosterone level is concomitant of male pubertal development, while the association of a high BC with testosterone level in the adult range, present in TF and male new-borns, seems characteristic of a peripheral inactivity of testosterone.
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Georges, P., Brijawi, A., Evain, D. et al. 94: Binding capacity of serum Testosterone-Estradiol Binding Globulin (TeBG) in males: Variations with age and pathological conditions. Pediatr Res 10, 885 (1976). https://doi.org/10.1203/00006450-197610000-00084
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DOI: https://doi.org/10.1203/00006450-197610000-00084
