Abstract
Biological somatomedin (SM) activity is usually assessed on sulfate incorporation in costal or pelvic cartilage. The present study evaluates the responsiveness of rabbit cultured growth plate chondrocytes. The specific radiosulfate incorporation into proteoglycans was measured after extracticn and purification on DEAE cellulose column. The distribution of the labelled proteoglycans between the cell pellet, the cell coat and the medium was evaluated. This method is very reproducible (inter-assay variation coefficient 3.4 %) and was applied to the bioassay of purified human plasma fractions with insulin-like activity (ILA) expressed as the ability to compete for 125-insulin on human placental membranes. CMC small peptides fraction had a potent sulfation activity, 4.56 × 104 dpm/flask (control 3.1.3 × 104) for 4 ng/ml ILA. RRA compared to insulin with 4.16 × 104 dpm/flask for 3000 ng/ml ILA RRA. This technique provides a new sensitive tool for bioassay on epiphyseal growth plate chondrocytes. (CMC fractions and ILA RRA in collaboration with B. Posner and H. Guyda).
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Corvol, M., Rappaport, R. 104: A sensitive technique for the measurement of the sulfation of purified proteoglycans in cultured epiphyseal growth plate chondrocytes. Pediatr Res 10, 887 (1976). https://doi.org/10.1203/00006450-197610000-00095
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DOI: https://doi.org/10.1203/00006450-197610000-00095
