Abstract
The administration of riboflavine (RF) is known to increase the activity of glutathione reduotase (GR). In this work the possibility of protection of red cell (rc) Hb from oxydative denaturation by the increased GR activity was investigated. RF (10mg daily) was given to 20 normal volunteers 10 males and 10 females aged 20-35 years, for one week. Before and after RF the following tests were performed. 1. Estimation of GR activity 2. Oxy-Hb,Met-Hb and sulf-Hb determination after 1-4h. incubation of rc suspensions or hemolysates with different concentrations of acetylphenyhydrazin (APH). Mean GR activity before RF was 57.71±23.63i.u/109rc and after RF 92.62±33.18i.u/109 rc. After RF the formation of Met-Hb was definetely decreased. There was a smaller decrease in the formation of Sulf-Hb. The protective effect of RF was more marked at 1h. of incubation and an APH:Hb ratio of 8:l. Unter these conditions the remaining Oxy-Hb was before RF (average values in 5 subjects) 88.5% in the rc suspensions and 83.8% in the hemolysates, while after RF it was 91.2% in the rc suspensions and 88.6% in the hemolysates. The difference was statistically significant for both, the rc suspensions (p<0. 01) and the hemolysates (p<0.001). It is concluded that RF protected to some degree Hb from oxydative, denaturation in vitro. This partial protection was very likely caused by the increased activity of GR after RF administration.
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Karaklis, A., Mantagos, S. & Pantelakis, S. 168: Protection of Hb from oxydative denaturation by riboflavin. Pediatr Res 10, 898 (1976). https://doi.org/10.1203/00006450-197610000-00159
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DOI: https://doi.org/10.1203/00006450-197610000-00159
