Abstract
Total protein, total phospholipids (PL) and phosphatidyl choline (PC) were measured in crude homogenate (CH), lamellar body (LB), mitochondrial (MT), and microsomal (MC) fractions of lung from the 28 and 30 day gestation fetus and adult Albino New Zealand rabbits. Gas chromatography of the fatty acid methylesters (FAME) of the PC in LB and MC was also done.
Total PL in LB increased 4-5 fold from 28 to 30 da gestation, but did not increase in MT or MC. PC (% of total PL) increased in LB from 60% at 28 days gestation to 84% at 30 days gestation, but did not increase in MT or MC. Gas chromatography of FAME in LB revealed a decrease in C14:0 from 7% at 28 days to 3.5% in adult, an increase of C16:0 from 54% to 58% and a decrease of C16:l from 12.4% to 6.5%. FAME in MC revealed a decrease of C16:0 from 40.2% at 28 days to 35.7% in the adult, a decrease of C18:l from 25.2% to 18.1%, and an increase of C18:2 from 9.7% to 14.7%. Other trends were evident.
This data supports the hypotheses that LB is the major storage organelle for PC at all gestations, increasing with maturity, and that maturational changes in fatty acids of PC also occurs.
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Gutcher, G., Tsao, F. & Zachman, R. SUBCELLULAR PHOSPHOLIPID MATURATION IN RABBIT LUNG. Pediatr Res 11, 572 (1977). https://doi.org/10.1203/00006450-197704000-01212
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DOI: https://doi.org/10.1203/00006450-197704000-01212
