Abstract
Under pentobarbital anesthesia, pulmonary and aortic indwelling catheters were successfully placed in 11 rats (Charles River CD; mean weight 256gm). Daily measurements were made of pulmonary artery pressure (Ppul), aortic pressure (Psys), heart rate (HR), and respiratory rate (RR) for an average of 2.5 day. to assure a stable preparation. The rats were then placed in a hypobaric chamber (air at 380 mmHg absolute) for a median period of 7 days (range 4-12). At the end of the hypoxic exposure, repeat measurements were made dreathing room air and after 5 min. breathing 10% O2. The animal, were then sacrificed and the right & left ventricles (RV, LV) were separated and weighed.
The Ppul rose 5 mm after 4 days and 17 mm average after 12 days exposure. No significant difference was found in the other parameters after hypoxia. Repeat measurement, in 10% O2 demonstrated no significant change from those made in air. Right ventricular hypertrophy was found in 10/11 rats exposed to hypoxia LV/RV - 2.6±.3, this was significantly decreased from controls (LV/RV = 3.8±.3) (p<.0025). We conclude, that during 12 days of hypoxic exposure pulmonary artery pressure rises continually and right ventricular hypertrophy occurs secondarily. This new technique of pulmonary artery catheterization via the Jugular vein and aortic cannulation may well be applicable to the study of a broad range of chronic physiological conditions.
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Gamble, W., Rabinovitch, M., Allard, P. et al. DEVELOPMENT OF PULMONARY ARTERY HYPERTENSION IN RATS UNDER HYPOXIC CONDITIONS, UTILIZING CHRONIC PULMONARY AND AORTIC CATHETERS. Pediatr Res 11, 390 (1977). https://doi.org/10.1203/00006450-197704000-00127
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DOI: https://doi.org/10.1203/00006450-197704000-00127
