Abstract
To examine macrophage receptor metabolism, rabbit pulmonary macrophages were exposed to trypsin (1mg/107 cells) for five minutes. This reduced C3-activated ingestion by 70%, but did not affect nonspecific or IgG-mediated ingestion. Trypsinized cells replaced their C3 receptors completely when incubated for four hours in media containing fresh or heated serum or 4% bovine albumin (BSA) at 37°, but not in buffered salt solutions with or without glucose and amino acids, gelatin solutions, or in any medium at 0°. Cycloheximide did not affect phagocytosis at a concentration of 2 ug/ml, but abolished 3H-leucine incorporation into protein, and prevented recovery of the C3 receptor of trypsinized cells. Rates of protein synthesis by macrophages were equivalent in the presence or absence of serum. We conclude that there are two independent requirements for receptor recovery: (1) endogenous protein synthesis, and (2) a heat-stable exogenous component present in serum and high concentrations of BSA.
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Shurin, S., Stossel, T. & Shannon, D. RECOVERY OF MACROPHAGE C3 RECEPTOR FUNCTION FOLLOWING SELECTIVE ENZYMATIC DESTRUCTION. Pediatr Res 11, 494 (1977). https://doi.org/10.1203/00006450-197704000-00746
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DOI: https://doi.org/10.1203/00006450-197704000-00746
