Abstract
We measured 60HF,tetrahydrocortisone (THE), tetrahy-drocortisol (THF) and free cortisol (FF) excretion by RIAs in 1 ml of urine.,Normal 60HF excretion in children is 0.28±.04 mg/d/m2(mean±SE) . We found no sex difference.The ratios(±SE) of these cortisol metabolites in normals,after ACTH (40×5d) in patients with Cushing's syndrome or disease (CSD) are:
Treatment with spironolactone caused a 6-fold mean increase in 60HF excretion, phenobarbital changed the 60HF/17OH ratio to 0.22±0.03, and Dilantin+phenobarbital altered the ratio to 0.45±0.1. Conclusions: 60HF excretion in relation to 17OH and FF is age dependent. 6OHF increased markedly in CSD and after ACTH. THE/THF ratio decreased after ACTH and in CSD. FF increased more after short-term ACTH than in CSD. Therefore ACTH and hypercortisolemia alter cortisol metabolism measurable by urinary metabolites. Drugs (spironolactone, phenobarbital,Dilantin) cause an increase in 6β-hydroxylation, a microsomal enzyme system.
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Saenger, P., Voccia, E., Gunczler, P. et al. 6βOH Cortisol (6OHF)as Indicator of Altered Cortisol (F) Metabolism. Pediatr Res 12, 1092 (1978). https://doi.org/10.1203/00006450-197811000-00068
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DOI: https://doi.org/10.1203/00006450-197811000-00068
