ORAL AND PARENTERAL IMMUNIZATION TO POVINE SERUN ALBUMIN IN THE PRESENCE OF PASSIVE ANTIBODY

Abstract

The effect of passive antibody (p.a.) on the antibody response to ingested Bovine Seriun Albumin (USA) was compared to intravenous (i.v.) and subcutaneous (s.c.) immunization. Antibody to BSA was measured by the Farr-technique and quantitatively expressed as μg BSA-N binding capacity/ml of serum (ABC 33). 18 rabbits, passively imunized to BSA fron a pool of high titered antiserum had a mean ABC 33 of 17.2. Four of these animals received no antigen (group I), 5 were fed 0.156 BSA in witer (gr. II), and 3 received 5 rag BSA s.c. (gr. III); 50 mg BSA was given i.v. to 3 aniraala (gr. IV) and 20 ag BSA i.v. to another 3 (gr. V). Control animals for groups II - V were actively immunized in the same way without prior administration of p.a. Catabolism of p.a. was similar in groups I and II (mean ABC 33 on day 25: gr: I 1.4 ± 0.5, gr. II 0.9 ± 0.5). Subsequently, serum anti-BSA in gr. II increased to 6,6 ± 5.7 on day 63 (control 3.6 ± 2.2, p> 0.05). In gr. III, mean ABC 33 was lowest on day 14 (2.2 ± 1.4) and increased to 51.7 ± 21.7 (control 9.4 ± 8.4, p<0.02). In gr. IV mean ABC 33 increased from day 7 (0.4 ± 0.7) to 3.1 ± 2.8 (control 0.3 ± C.5, P<0.05). No active immunity developed in gr. V (control 0.17 ± 0.21). Confirming previous data, p.a. enhanced or suppressed parenteral immunization depending on routes of immunization and antigen/antibody ratios. In contrast, p.a. did not influence oral immunization and its catabolism was not affected by the ingested antigen.

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