Abstract
Tetracycline-induced fatty liver(TFL)in animals and humans produces clinical and biochemical features similar to idiopathic fatty liver of pregnancy and Reye's syndrome(RS). The light microscopic features of hepatic panlobular microvesicular steatosis with no necrosis or inflammation is common to TFL and RS. We measured hepatic urea cycle enzymes carbamyl phosphate synthetase(CPS),ornithine transcarbamylase(OTC),and arginase(ARG) in 20 day old weanling rats after administration of tetracycline 40 mg/kg and 100mg/kg intraperitoneally daily for 7 days. Tetracycline was infused daily intravenously to another group of 20 day old weanling rats for 3 days. Control rats were injected IP and IV with similar doses of ascorbic acid. Hepatic CPS,OTC and ARG was determined in the IP and IV tetracycline-injected rats and the ascorbic acid-injected(control)rats. Mean CPS values were 39.0±9.55 nanomol.cit./mg.prot/min,OTC 651.1±337.6 nanomol.cit/mg.prot./min. and ARG 3143.3±1954.2 nanomol.urea/mg.prot/min. In the TFL group the values for control animals were 35.5(CPS), 564.6(OTC), and 1762.7(ARG)respectively. E/M features revealed normal mitochondria with abnormal peroxisomes. TFL in weanling rats produced no depression of urea cycle enzyme activities and no mitochondrial ultrastructural abnormalities resembling RS. Further studies are in progress to define the biochemical and ultrastructural correlates of TFL as an animal model of RS.
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Lansky, L., Hong, L. & Hug, G. 451 UREA CYCLE ENZYME ACTIVITIES AND ULTRASTRUCTURAL FEATURES OF TETRACYCLINE-INDUCED FATTY LIVER. Pediatr Res 12 (Suppl 4), 439 (1978). https://doi.org/10.1203/00006450-197804001-00456
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DOI: https://doi.org/10.1203/00006450-197804001-00456
