Abstract
By screening of 400 sera of pregnant women in an indirect immunofluorescent test with T-lyraphocytes and several EBV transformed B-cell lines, 3 sera with B-cellalloantibodies were detected. These sera did not react with T-cell-leukemia line Molt-4 and JM ( 1 ), but were reactive with ‘Null’-cell-leukemia line KH-3.
For the purpose of a direct immunofluorescent test, the globulinfraction of these sera were first fluorescinated and then incubated simultaneously with a rhodamine-conjugated antiimmunoglobulin with bone marrow of children with ALL.
Two different types of cell population were observed:
1) one population with double fluorescence (probably B-lymphocytes and monocytes)
2) exclusively fluorescein-stained cells (predominantly blasts)
Even though the latter population does rarely occur in normal non-leukemic bone marrow, the method still seems to be helpful for the determination of the quality of a hematological remission.
(1) Int. J. Cancer 191621 (1977)
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Schwenk, H., Schneider, U. & Becker, H. Detection of lymphoblasts from common-type ALL in the bone marrow with a double marker method. Pediatr Res 12, 71 (1978). https://doi.org/10.1203/00006450-197801000-00069
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DOI: https://doi.org/10.1203/00006450-197801000-00069
