Abstract
In order to evaluate the cellular basis of the synergistic effect of LPS and ConA on in vitro IgM production their effect on cell proliferation was studied under identical experimental conditions in normal subjects and in patients with X-linked agammaglobulinaemia (XLA). While cell viability was not affected by any dose of LPS, increasing doses of LPS progressively reduced thymidine incorporation on day 3 in ConA stimulated cultures from 10 normal subjects; on day 5 inhibition of DNA synthesis was still present except in those doses of LPS eliciting maximum IgM production where a synergistic effect became evident. In 4 patients with X-LA synthesis of IgM was not induced by any combination of PWM, ConA or LPS. The pattern of thymidine incorporation was however similar to that observed in normal controls indicating that the inhibitory effect of LPS on cell proliferation is not dependent on the presence of functional B cells.
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Maccario, R., Nespoli, L., Monafo, V. et al. 36 INTERACTION OF LPS AND CONA IN HUMN LYMPHOCYTE ACTIVATION. II: EFFECT ON CELL PROLIFERATION. Pediatr Res 13, 954 (1979). https://doi.org/10.1203/00006450-197908000-00052
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DOI: https://doi.org/10.1203/00006450-197908000-00052
