Abstract
Purpose of study. Earlier we have shown that K antibodies are important for protection against acute pyelonephritis in rats. So far, only whole bacteria have been used to induce such protective antibodies, as isolated E.coli capsular antigen is non immunogenic. The aim of the present investigation was to study if a protective effect could be shown after immunization with isolated E.coli K antigen conjugated to a carrier substance.
Methods. E.coli K13 antigen, conjugated to a carrier substance to make it immunogenic, was used for immunization of Sprague-Dawley rats. Acute, ascending pyelonephritis was then induced in immunized and non immunized rats by intravesical injection of 108 E.coli O6K13H1 bacteria.
Results. Rats immunized with two subcutaneous or two intraperitoneal injections (2.5 μg/injection/rat) had less often acute pyelonephritis after the bacterial injection, than the non immunized controls (50% compared to 87%; p<0.01).
Conclusion. The present investigation has shown for the first time that isolated E.coli K13 antigen, conjugated to a carrier substance, can be used for prophylactic immunization against acute pyelonephritis.
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Kaijser, B., Larsson, P. & Schneerson, R. PROTECTION AGAINST ACUTE, ASCENDING PYELONEPHRITIS CAUSED BY E.COLI IN RATS USING ISOLATED CAPSULAR ANTIGEN CONJUGATED TO A CARRIER SUBSTANCE. Pediatr Res 14, 980 (1980). https://doi.org/10.1203/00006450-198008000-00040
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DOI: https://doi.org/10.1203/00006450-198008000-00040
